Additional file 4: of A parental requirement for dual-specificity phosphatase 6 in zebrafish

2018-03-15T05:00:00Z (GMT) by Jennifer Maurer Charles SagerstrĂśm
Nucleotide and predicted amino acid sequence of mutant alleles. A, B. Nucleotide and predicted amino acid sequence of dusp6 (A) and dusp2 (B) mutant alleles. Genomic DNA was amplified using primer pairs dusp6-1/dusp6-2 and dusp2-1/dusp2-3 (See Fig. 2a and Additional file 2). Uppercase nucleotides indicate the coding sequence and lowercase nucleotides represent the UTR. Green and red nucleotides indicate translation start and stop sites, respectively. Ellipses (...) indicate the continuation of wild type sequences. Dashes (--) indicate deletions and blue nucleotides indicate insertions in the mutant alleles. Predicted protein sequences are indicated in one letter IUPAC code with purple highlighting the rhodanese homology domain and green highlighting the catalytic domain. Asterisks denote the stop codon. Orange residues are predicted to be encoded by the mutant alleles before encountering a stop codon. C. Transcripts are produced in dusp6 mutant embryos. cDNA was amplified from homozygous mutant dusp6 embryos by end-point PCR using the same primers as in A. Sequencing of these transcripts identified the same lesions detected in genomic DNA in A. Dashed lines indicate where gel was cut. (TIFF 1730 kb)