Additional file 4: of A map of gene expression in neutrophil-like cell lines

(a) PLB-985 cells were differentiated into a neutrophil-like state by culturing in media supplemented with 1.3% DMSO, 2% Nutridoma and the indicated percentage of FBS. Cells were stained with FLPEP and measured by cytometry. (b-c) PLB-985 cells were differentiated into a neutrophil-like state by culturing in media supplemented with 1.3% DMSO 0.5% FBS and either 2% Nutridoma or 30 ng/mL G-CSF for 6 days. Cells were stained with anti-CD11b (b) or with FLPEP (c) and measured by cytometry. Cytometry data was analyzed using MATLAB in all the experiments. Data for the undifferentiated cells and those differentiated with DMSO or DMSO + Nutridoma is the same as that shown in Fig. 2. (PDF 158 kb)