Additional file 4: Figure S4. of Systematic comparison of small RNA library preparation protocols for next-generation sequencing

Histograms representing the percentage of the total numbers of raw reads corresponding to RNA(OMe)1–6 with (A) the TruSeq protocol (TS1), (B) the NEXTflex protocol (Nf2), and (C) the SMARTer protocol (S). Green bars represent the results obtained with the standard numbers of PCR cycles (11 cycles for TS1, 14 cycles for Nf2, and 7 cycles for S), blue bars represent 50 additional cycles of PCR, and red bars represent the standard number of PCR cycles but using AMV reverse transcriptase instead of Superscript II for cDNA synthesis. (PDF 1.01 mb)