Additional file 4: Figure S4. of Somatic cell selection for chlorsulfuron-resistant mutants in potato: identification of point mutations in the acetohydroxyacid synthase gene

Nucleotide sequence of the mutated potato AHAS coding region. The two nucleotides highlighted in red are the point mutations identified in the somatic cell selection experiments from cell colonies CR06, CR27. The first mutation, the highlighted residue at nucleotide 556 was originally C and was converted to a T, which changes the 186th amino acid from a proline into a serine residue. The second highlighted residue was identified to have a mutation at position 1687, where a T residue was converted to A, which changed the 563rd amino acid from tryptophan into an arginine residue. (DOCX 12 kb)