Additional file 4: Figure S4. of Rapid, precise quantification of bacterial cellular dimensions across a genomic-scale knockout library

Morphological analysis of the Keio collection reveals correlations between cell width and intracellular width variability. Contours from cells from each Keio deletion strain were extracted from images acquired from the NBRP repository and used to compute the mean width and width profile across each cell. For each cell, we then computed the standard deviation of the width profile divided by the mean width to obtain the intracellular width variability. White circles and error bars were obtained by binning strains by mean width; blue lines are the fit to binned averages. R is Pearson’s correlation coefficient; p-value was computed with Student’s t-test. (PDF 111 kb)