Additional file 4: Figure S4. of MicroRNA-1296 inhibits metastasis and epithelial-mesenchymal transition of hepatocellular carcinoma by targeting SRPK1-mediated PI3K/AKT pathway

SRPK1 faciliates migration, invasion and EMT progression of HCC cells. (A) HCCLM3 cells that were transfected with SRPK1 siRNA or control siRNA were detected by immunoblotting. (B) SRPK1 silencing notably restrained migration and invasion of HCCLM3 cells. (C) SRPK1 was overexpressed by plasmid transfection and confirmed by western blotting in Hep3B cells. (D) SRPK1 restoration enhanced the migratory and invasive abilities of Hep3B cells. (E) SRPK1 knockdown led to increase of E-cadherin expression and decrease of N-cadherin and Vimentin in HCCLM3 cells. (F) SRPK1 overexpression promoted the EMT progression of Hep3B cells. *P < 0.05. (TIFF 3508 kb)