Additional file 4: Figure S4. of Agonists and knockdown of estrogen receptor β differentially affect invasion of triple-negative breast cancer cells in vitro

Proliferation of MDA-MB-231 and HS578T breast cancer cells after knockdown of ESR2 gene expression. The day after transfection with negative control siRNA or ESR2 siRNA, cells were seeded in DMEM/F12 plus 10% FSC in triplicates and relative numbers of viable cells were measured on day 0, 2, 3 and 4 using the Cell Titer Blue assay (Promega) as described in the Materials and Methods section. Values are expressed in percent of proliferation of mock-transfected cells (n = 3). *p < 0.05 vs. mock-transfected cells (Kruskal-Wallis H-test with Bonferroni post-hoc test). (JPG 41 kb)