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Additional file 4: Figure S3. of Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
figure
posted on 2017-06-08, 05:00 authored by Samantha de Witte, Ana Merino, Marcella Franquesa, Tanja Strini, Johanna van Zoggel, Sander Korevaar, Franka Luk, Madhu Gargesha, Lisa O’Flynn, Debashish Roy, Steve Elliman, Philip Newsome, Carla Baan, Martin HoogduijnEx vivo experimental scheme. UcMSC were pre-treated for 3 days. Medium was then refreshed and the cells were left overnight in the incubator. The following day, livers were collected from healthy C57BL/6 mice and directly after isolation cut into slices (diameter = 1 cm and thickness = 150 μm) and placed in the wells on top of the pre-treated ucMSC in the presence of LPS. The liver slices were left overnight in the incubator on a shaker (50 rpm). After 24 hours, supernatant and liver slices were harvested. (TIF 4600 kb)
