Additional file 4: Figure S3. of Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease

Ex vivo experimental scheme. UcMSC were pre-treated for 3 days. Medium was then refreshed and the cells were left overnight in the incubator. The following day, livers were collected from healthy C57BL/6 mice and directly after isolation cut into slices (diameter = 1 cm and thickness = 150 μm) and placed in the wells on top of the pre-treated ucMSC in the presence of LPS. The liver slices were left overnight in the incubator on a shaker (50 rpm). After 24 hours, supernatant and liver slices were harvested. (TIF 4600 kb)