Additional file 4: Figure S3. of Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation

In vitro cartilage formation by pellet culture of synovial MSCs. Synovial MSCs before and 48 h after preservation in human serum at each temperature were pelleted after centrifugation, then cell pellets were cultured in chondrogenic medium for 21 days. Spheroids of cartilage derived from synovial MSCs were observed in cells preserved at 4 and 13 °C, while synovial MSCs were not condensed in cells preserved at 37 °C. Dot line shows cell pellet. (PDF 332 kb)