Additional file 4: Figure S3. of 2.7 million samples genotyped for HLA by next generation sequencing: lessons learned

Average primer dimer rates for different amplicons, genes, PCR setups and reaction volumes. PCR setups are a 1-PCR 4-primer setup vs. a standard 2-PCR 2-primer setup. Differences in reaction volumes are denoted by PCR (10 μl) and Fluidigm (10 nl). Only amplicons with an average PD rate > 0.2% are labelled in the plot. The y-axis is square root scaled to enhance readability. (PDF 5 kb)