Additional file 4: Figure S2. of The repressive effect of miR-148a on Wnt/β-catenin signaling involved in Glabridin-induced anti-angiogenesis in human breast cancer cells

MDA-MB-231 cells were pretreated with 0, or 20 μM GLA for 48 h, then the media was removed, the cells were washed with 1× PBS, followed by replacement with fresh media with 1% FBS for 24 h. (A)The expression of miR-148a was analyzed by qRT-PCR (mean ± SD, n = 3). (B-C) Western blot analyses the relative protein levels of Wnt1, β-catenin, and non-phospho (active) β-catenin (mean ± SD, n = 3). (D) The ELISA was used to detect the secretion of VEGF (mean ± SD, n = 3); **P < 0.01 and ***P < 0.001 compared with the control media or cells. (DOCX 322 kb)