Additional file 4: Figure S2. of Hypermethylation and loss of retinoic acid receptor responder 1 expression in human choriocarcinoma

DNA methylation of choriocarcinoma cell lines following AZA treatment. Swan71 (A), Jeg-3 (B), JAR (C) and BeWo (D) cells were treated with DMSO or AZA for 72 h and DNA methylation of CpG region 1 was measured by pyrosequencing (n = 3, respectively). Each circle represents one biological replicate. The bars represent the median with 95% CI. Significance is marked by asterisks (** p < 0.005). (TIFF 1049 kb)