41021_2015_24_MOESM4_ESM.ppt (206.5 kB)
Additional file 4: Fig. S4. of Genomic integration of lambda EG10 transgene in gpt delta transgenic rodents
presentation
posted on 2015-12-01, 05:00 authored by Kenichi Masumura, Yasuteru Sakamoto, Wakako Kumita, Masamitsu Honma, Akiyoshi Nishikawa, Takehiko NohmiPCR analysis of the type of junctions between lambda EG10 copies. PCR primers were set at both ends of lambda EG10 to amplify the connecting region of two EG10 copies. The genomic DNA of gpt delta mice and rats was used as the PCR template. PCR was performed as described in genotyping section in the Materials and Methods. (PPT 206 kb)