Additional file 3: of Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union

Figure S3. Immunohistochemistry of grafted hypocotyl sections – extensins (JIM12 and LM1 epitopes) and AGPs (JIM13, JIM8, and LM2 epitopes). A – epitope present in some of the cortical cells (full arrow) and graft union area (arrowheads), intensive fluorescence signal detected in the outer periclinal cell walls and cuticle of the epidermis (arrow); inset: intensive fluorescence signal detected in the outer periclinal cell walls and cuticle of the epidermis (arrow). B – epitope detected in the cell wall (arrow) and on the outside of the cell (arrowhead). C – epitope present in the cytoplasmic compartments of cortical cells near the graft union area (arrow). D – occurrence of epitope in the cells of the regenerated vascular bundle (arrows), in some endodermal cells (arrowhead), and peripheral cells of the graft union (inset: arrowhead), no fluorescence signal detected on the cell surface (full arrow). E – epitope present in the cytoplasm and/or plasmolemma of the graft union cells located peripherally (arrowheads), no fluorescence signal detected on the cell surface (arrow). F and inset – weak labeling in the cytoplasmic compartments of the peripheral cells (arrowheads), no fluorescence signal detected on the cell surface (arrows). c Calcofluor White, ep epidermis. Scale bars: A, D and D inset, and F = 50 μm; B, C, E, A inset, and F inset = 10 μm (JPG 2588 kb)