Additional file 3: of Transcriptomic and epigenetic profiling of ‘diffuse midline gliomas, H3 K27M-mutant’ discriminate two subgroups based on the type of histone H3 mutated and not supratentorial or infratentorial location

Figure S1. A. Principal component analysis of microarray GE profiling of 119 high grade gliomas as presented in Fig. 1b colored by mutational histone H3 status. Five pontine WT tumors that also harbor a H3K27 trimethylation loss by IHC are highlighted with red arrowheads. B- The gene expression data of the 120 genes associated with the highest standard deviation (n = 120 genes) were used for k-means analysis (k = 2) and the results represented in the same PCA as in panel A. The samples were color-coded according to k-means results, symbols reflect the histone H3 mutational status and their location are indicated in the plot. C-D. t-SNE analysis of the GE profiles of 119 pediatric high-grade gliomas using the genes associated with the highest standard deviation (n = 120 genes). The tumors were color-coded according to their location (A) or mutational histone H3 status (B) as described in Fig. 1. E- Overlapping of the gene lists resulting from differential analysis between: H3-K27M and wild-type tumors, H3-K27M and G34R tumors, H3-G34R and wild-type tumors, H3.1 K27M and H3.3-K27M tumors (adjusted p-value< 0.01). (PDF 224 kb)