Additional file 3: of The lncRNA UCA1 promotes proliferation, migration, immune escape and inhibits apoptosis in gastric cancer by sponging anti-tumor miRNAs

Figure S3. UCA1 interact with miR-26a, miR-26b, miR-193a and miR-214 in GC cells and regulate their targets expression. (A) Cell extracts were incubated with biotin labelled UCA1 probe, with biotin labelled sequence scrambled DNA oligo as control. Hybridized material was captured with magnetic streptavidin beads. Beads were subjected to RNA extraction followed by RT-qPCR to quantify miRNAs levels. (B) Fluorescence in situ hybridization to identify the colocalization between UCA1 and miRNAs. (C) miRNAs expressions were quantified by RT-qPCR in UCA1 knockdown cells. (D) The protein level of TAK1(miR-26a/b target), KRAS(miR-193a target) and FGF9 was examined by immunoblotting in UCA1 overexpression or knockdown GC cells (TIF 14 kb)