Additional file 3: of Systemic overexpression of SQSTM1/p62 accelerates disease onset in a SOD1H46R-expressing ALS mouse model

Figure S3. SQSTM1-HA positive aggregates in the anterior horn of lumbar spinal cord. a-c Representative images of double immunostaining with Ubiquitin (green) and HA (SQSTM1-HA; red) in the lumbar spinal cord (L4–5) from wild-type (WT), SQSTM1 (SQSTM1), SOD1 H46R (H46R) and SQSTM1;SOD1 H46R (SQSTM1;H46R) mice at 16 weeks of age (wk) (a), 22 wk. (b), and end-stage (H46R and SQSTM1;H46R) or 28 wk. (WT and SQSTM1) (c). The nuclei were counterstained with DAPI (blue). Scale bars = 50 μm. Ubiquitin-positive aggregates and SQSTM1-HA aggregates were observed in the anterior horn of SQSTM1;SOD1 H46R (SQSTM1;H46R) mice at 22 wk. and end-stage. Arrows indicate that ubiquitin-positive aggregates colocalizing with SQSTM1-HA. (PDF 1718 kb)