Additional file 3: of Structural and functional characterization of protein–lipid interactions of the Salmonella typhimurium melibiose transporter MelB

Figure S3. Galactoside binding. A Trp→ D2G FRET assay was used to detect the binding of the [13C, 15N]-labeled MelBSt after reconstituted into proteoliposomes as described in the Methods. On the time trace set at an excitation wavelength of 290 nm and emission wavelength of 490 nm, D2G at 10 μM was added into the MelBSt liposome samples at 60-s time point, and melibiose at a saturation concentration or equal volume of water was further added into the solution at 120-s time point. (PDF 701 kb)