Additional file 3: of Redirecting differentiation of mammary progenitor cells by 3D bioprinted sweat gland microenvironment

Figure S3. Differentiation of mammary progenitor cells (MPCs) in two-dimensional (2D) cultured environment. (a) Immunofluorescence staining of ATP1a1 of induced cells cultured in 2D cultured environment without mouse sweat gland-extracellular matrix (SG-ECM) proteins. (scale bar, 50 Îźm). (b) Immunofluorescence staining of ATP1a1 of induced cells cultured in 2D cultured environment with mouse SG-ECM proteins. (scale bar, 50 Îźm). (c) Gene expression of ATP1a1 of different groups. The group of SG is positive control. Data were presented as mean Âą standard deviation (n = 3). In the statistical analysis, one-way ANOVA was used to measure the difference between these three groups. In each group comparison, SNK-q test was used. **p < 0.01. (JPG 47 kb)