12864_2017_4371_MOESM3_ESM.pdf (87.05 kB)
Additional file 3: of Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
journal contribution
posted on 2017-12-21, 05:00 authored by Jian Zhong, Zhenqing Ye, Samuel Lenz, Chad Clark, Adil Bharucha, Gianrico Farrugia, Keith Robertson, Zhiguo Zhang, Tamas Ordog, Jeong-Heon LeeChIP enrichment analyzed by qPCR. The ChIP DNA was analyzed by qPCR in a region of constitutively active chromatin region (GAPDH-TSS, H3K4me3-positive), a developmentally repressed region (MYT1-TSS, H3K27me3-positive), and an intergenic region (C19, H3K4me3- and H3K27me3-negative) from HeLa cells. Enrichment in the tested loci is shown as the percentage of input. (PDF 87Â kb)