Additional file 3: of Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome system

Figure S2. Confirmation of pMMBL101 (A) PCR analysis using randomly selected tmc gene primers (B) Enzyme mapping using various restriction enzyme. C, uncut pMMBL101; 1, EcoRI; 2, EcoRV; 3, NdeI; 4, HindIII; 5, XbaI-digested pMMBL101; M, Îť-HindIII DNA ladder.