Additional file 3: of Precise cloning and tandem integration of large polyketide biosynthetic gene cluster using Streptomyces artificial chromosome system
2015-09-16T05:00:00Z (GMT)
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Figure S2. Confirmation of pMMBL101 (A) PCR analysis using randomly selected tmc gene primers (B) Enzyme mapping using various restriction enzyme. C, uncut pMMBL101; 1, EcoRI; 2, EcoRV; 3, NdeI; 4, HindIII; 5, XbaI-digested pMMBL101; M, Îť-HindIII DNA ladder.