Additional file 3 of Parkinson’s disease-related Leucine-rich repeat kinase 2 modulates nuclear morphology and genomic stability in striatal projection neurons during aging

Additional file 3: Figure S1. No alteration of nuclear size in midbrain dopaminergic neurons and hippocampal neurons of 12-month-old Lrrk2−/− mice. a-c Co-staining of Lamin B and TH in SNc and VTA neurons of 12-month-old Lrrk2+/+ and Lrrk2−/− mice (a). Scale bar, 20 μm. The areas of soma and nuclei in SNc neurons were measured from five 12-month-old Lrrk2+/+ and Lrrk2−/− mice (b). N = 5 mice per genotype, about 30 neurons counted per animal. Conditional logistic regression test, no statistically significant difference was identified. The areas of soma and nuclei in VTA neurons were measured from five 12-month-old Lrrk2+/+ and Lrrk2−/− mice (c). N = 5 mice per genotype, about 30 neurons counted per animal. Conditional logistic regression test, no statistically significant difference was identified. d-f Co-staining of Lamin B and MAP2 in hippocampal dentate gyrus (DG) neurons of 12-month-old Lrrk2+/+ and Lrrk2−/− mice (d). Scale bar, 20 μm. The area of nuclei in DG neurons was measured from three 12-month-old Lrrk2+/+ and Lrrk2−/− mice e). N = 3 mice per genotype, about 50 neurons counted per animal. Conditional logistic regression test, no statistically significant difference was identified. The circularity of nuclei in DG neurons was measured from three 12-month-old Lrrk2+/+ and Lrrk2−/− mice (f). N = 3 mice per genotype, about 50 neurons counted per animal. Conditional logistic regression test, no statistically significant difference was identified.