Additional file 3: of Most of anti-glycolipid IgG-antibodies associated to neurological disorders occur without their IgM counterpart

Figure S2. Absence of anti-glycan IgM reactivity in IgG-reactive sera is not due to IgG interference. Randomly selected discordant serum samples were subjected to whole IgG fraction removal using protein G-affinity columns. Briefly, serum pH was adjusted by adding 1/10 volume of 1 M Tris buffer (pH 8). After filtration, the serum was passed through Sephadex columns with covalently bound Protein G (1 ml Protein G / 1 ml serum), with subsequent washes using 100 mM Tris buffer (pH 8.0). Examples for whole serum samples (“Before Protein G”) and non-adsorbed fractions (“After Protein G”) assayed for IgM and IgG using HPTLC-I are shown. (TIF 3814 kb)