Additional file 3 of Mechanical factors tune the sensitivity of mdx muscle to eccentric strength loss and its protection by antioxidant and calcium modulators

Additional file 3: Figure S3. Ex vivo and in vivo force/torque frequency curve for mdx muscle. (A) The frequencies used in the ex vivo study of the EDL were 0, 35 and 120 Hz, which represent muscle lengthening without stimulation, that which elicited force half-way between a twitch and maximal tetanus, and the frequency required to generate a maximal tetanic contraction (381 ± 4 mN), respectively. N = 4. (B) Torque-frequency analysis of the anterior crural muscles using a 40° angle change at 2000°/s. Stimulation frequencies were 0, 52, 71, 93 and 150 Hz which represent ankle rotation without stimulation, and frequencies required to generate 50, 75, 90 and 100% of the difference between a twitch (1.04 ± 0.04 mN·m) and tetanus (2.84 ± 0.1 mN·m). N = 8.