Additional file 3: of Long non-coding RNA NEAT1-modulated abnormal lipolysis via ATGL drives hepatocellular carcinoma proliferation

Figure S1. ATGL mRNA was aberrantly expressed in HCC tissues and mediates lipolysis in HCC cells. A. Real-time PCR analysis of HSL, MAGL and ATGL expression in five pairs of HCC and matched non-tumor tissues. B. Transfection efficiency of ATGL and sh-ATGL as detected by western blot. C. Overexpression of ATGL increased intracellular FFA and DAG levels in Huh7 and HepG2 cell lines. D. ATGL knockdown (or treatment with Atglistatin) reduced intracellular FFA and DAG levels in HCCLM3 and SK-Hep-1 cell lines. Data are expressed as mean ± SD of three independent experiments. Statistical significance was concluded at **P < 0.01, ***P < 0.001. (TIF 436 kb)