Springer Nature
Browse
12943_2018_821_MOESM3_ESM.docx (109.01 kB)

Additional file 3: of Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer

Download (109.01 kB)
journal contribution
posted on 2018-03-10, 05:00 authored by Kuo-Wang Tsai, Yi-Hao Lo, Hsuan Liu, Chung-Yu Yeh, You-Zuo Chen, Chao-Wen Hsu, Wei-Shone Chen, Jui-Ho Wang
Figure S3. Examination of the subcellular fractionation localization of Linc00659 in CRC cell lines. After nuclear and cytosolic separation, total RNA from Lovo, HCT116, HT29, SW620 and DLD-1 cells underwent RT and real-time PCR. GAPDH was used as a cytosol marker (A) and U6 was used as a nucleus marker (B). (C) RNA expression levels of Linc00659 candidates in the nucleus and cytoplasm were measured by real-time PCR, respectively. CRC, colorectal cancer; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PCR, polymerase chain reaction. (DOCX 109 kb)

Funding

Ministry of Science and Technology, Taiwan

History