Additional file 3: of Hypoxia-induced Slug SUMOylation enhances lung cancer metastasis

Figure S3. The Slug protein levels reflect its SUMOylated levels. To correlate the protein expression levels with the levels of SUMOylation, we subcutaneously injected KEK293 cells overexpressing Slug/vector control or Slug/HA–Ubc9 into mice. Tumor tissues were removed at 42 days after tumor injection and then lysed with tissue protein extraction reagent contained proteinase inhibitors and NEM. Subsequently, the samples were subjected to immunoprecipitation with an anti-Slug antibody prior to immunoblotting with the indicated antibodies. β-actin was used as the internal control. The asterisk and arrowhead indicate Slug modified and not modified by ubiquitin, respectively. (PDF 26 kb)