Additional file 3: of Human neural stem cell-derived neuron/astrocyte co-cultures respond to La Crosse virus infection with proinflammatory cytokines and chemokines

Figure S3. Innate immune responses to inflammatory stimuli in neuron/astrocyte co-cultures. RT-PCR and BioPlex assays were performed to determine the cytokine and chemokine responses of neuron/astrocyte co-cultures. (a) Co-cultures were treated with either mock, Poly I:C, or heat inactivated LACV and at 48 HPI assessed for changes in gene expression for selected cytokines/chemokines via qRT-PCR. Values are reported as fold change relative to mock treatment normalized to 18S RNA. (b) Supernatant was collected and assayed for changes in selected cytokine/chemokine secretion via BioPlex assay. * P < 0.5, **P < 0.01, ***P < 0.001, ****P < 0.0001. (PDF 207 kb)