Additional file 3 of High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers

Additional file 3: : Suppl. Figureure 3: Overrepresentation of mitochondrial proteins in soleus type IIa fibers reflects increased numbers of mitochondria. A) Volcano plot comparing protein fold-changes (log2-scale) in soleus type I and type IIa fibers (Table S3). Colored circles reflect mitochondrial proteins (blue, MitoCarta2.0-annotated) and OXPHOS complex members. B) The same volcano plot as in A) but showing normalized values for mitochondrial annotated proteins. Respiratory chain complex I, III, IV and V members were selected, and mean intensities were calculated for type I and IIa samples separately. A correction factor was calculated by correlating the average value of type I complex members to the average value of type II members. The mean relative intensity in type I fibers was 0.745 (compared to type IIa).