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Additional file 3: of Germline mutation within COL2A1 associated with lethal chondrodysplasia in a polled Holstein family
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posted on 2017-10-10, 05:00 authored by Sina Reinartz, Hartmut Mohwinkel, Christian Sürie, Maren Hellige, Karsten Feige, Deborah Eikelberg, Andreas Beineke, Julia Metzger, Ottmar DistlGenotyping of all three bulldog calves, a sperm sample of their sire Energy P, the dams of the bulldog calves and an unrelated control animal using a PCR restriction fragment polymorphism (PCR-RFLP). Samples with fragment sizes of 394 bp, 79 bp, 51 bp and 28 bp represent the heterozygous missense variant g.32476082G > R. (TIFF 538 kb)