Additional file 3: of Germline mutation within COL2A1 associated with lethal chondrodysplasia in a polled Holstein family

Genotyping of all three bulldog calves, a sperm sample of their sire Energy P, the dams of the bulldog calves and an unrelated control animal using a PCR restriction fragment polymorphism (PCR-RFLP). Samples with fragment sizes of 394 bp, 79 bp, 51 bp and 28 bp represent the heterozygous missense variant g.32476082G > R. (TIFF 538 kb)