Additional file 3: of Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins

Figure S3. Comparison of morphological and electrophysiological properties of layer 4 neurons between P13–16 and P18–20 chimeric mice. (A) Traces of spiny stellate cells sampled from P13–16 wild-type chimeric mice (n = 9 cells, n = 8 barrels, n = 3 mice). Scale bar: 100 μm. (B–D) Comparison of the dendritic morphology between P13–16 (n = 9) and P18–20 (n = 16) chimeric mice. No significant differences in the total dendritic length (P = 0.26, t test, B) or the number of branches (P = 0.20, C) were observed, but there was a difference in the number of intersections near soma (*P = 0.046, D). A bar indicates the mean (B, C). Data presented as mean ± SEM in D. (E–G) Comparison of the GFP-negative neurons (N-cell) or GFP-positive neurons (P-cell) between P13–16 and P18–20 chimeric mice in the resting potential (P = 0.30 for GFP-negative neurons, P = 0.36 for GFP-positive neurons, t test, E), firing threshold (P = 0.44 for GFP-negative neurons, P = 0.20 for GFP-positive neurons, F), and input resistance (***P = 0.0007 for GFP-negative neurons, **P = 0.003 for GFP-positive neurons, G). Number of analyzed cells is shown above each box-and-whisker plot (median, 25th to 75th percentiles, minimum to maximum) (E–G). (PDF 636 kb)