Additional file 3: of ErbB2-driven downregulation of the transcription factor Irf6 in breast epithelial cells is required for their 3D growth

Figure S2. p63-specific siRNAs downregulate Irf6 in MCF10A cells in 3D culture. MCF10A cells transfected with 100 nM control RNA (cRNA) or p63-specific siRNA (p63siRNA) 14 or 15 were kept in 3D culture for 3 h and assayed for Irf6 expression by Western blotting. β-actin was used as a loading control in one experiment, and α-tubulin was used as a loading control in another independent experiment. Films were scanned, and densitometric analysis of the resulting digital images was performed. Irf6 protein levels were normalized to those of the loading controls. The data represent the average of two independent experiments plus the SD. * p < 0.05. (PPT 53 kb)