Additional file 3 of Engineering of Trichoderma reesei for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3

Additional file 3: Figure S2. The verification of the transformants. (A-B) PCR amplification results of the A734 (A) and A723 (B) transformants. F were obtained using the primer pair ace3-CF/D70-4. R were obtained using the primer pair HG3.6/ace3-CR (Additional file 8: Table S1). (C) Schematic for identification of single-copy DNA integration in transformants genome. Primer pairs (ace3-T1/ace3-T2 and ace3-T3/ace3-T4 list in Additional file 8: Table S1) showed in red were used to identify the copy number of integrated genes. (D) The verification of copy numbers for A734 and A723 transformants by qPCR. The genome of QM6a is used as a reference with the single copy of native ace3. (E) A723 and A734 transformants were confirmed by DNA sequencing. The diagnostic PCR amplification results were sequenced. Black boxes represent the missense mutation loci in the sequence of ace3. Red underline represents the DNA sequence of the 11 truncated amino acids. The green dotted underlines indicate the stop codons.