Additional file 3: of ETV4 transcription factor and MMP13 metalloprotease are interplaying actors of breast tumorigenesis

Figure S1. Validation of the overexpression of ETV4 and MMP13 in MCF10A cells. a and b Relative ETV4 mRNA (a) and MMP13 mRNA (b) expression in the MCF10A-Ctrl and MCF10A-ETV4 cells determined by real-time PCR and normalized to cyclophilin A levels. mRNA expression in MCF10A-Ctrl cells was arbitrarily = 1. Error bars indicate SD. *P ≤ 0.1. c and d Western blot analysis of ETV4 protein expression (61 kDa) (c) and MMP13 protein expression (60 kDa) (d) in the MCF10A-Ctrl and MCF10A-ETV4 cells. GAPDH expression served as the loading control. (PDF 100 kb)