Additional file 3: of E90 subunit vaccine protects mice from Zika virus infection and microcephaly

Figure S2. Vaccination of female mice protects offspring from early ZIKV infection. a. Schematic for immunizations and mating of CD1 (ICR) female mice with ZIKV challenge in early pregnancy. Mice vaccinated with E90 or PBS were mated at D28 and treated with anti-Ifnar1 antibody at E5.5. Mice were infected with ZIKV virus (GZ01 strain) at E6.5. b. Viral loads in placenta at E16.5 were measured by qRT-PCR. n = 16 for each group. All data are means ± SEM. Student’s t-test. ***p < 0.001. c. Condition of embryos in the PBS group after ZIKV infection at E18.5 (arrows indicate physical deformities, also shown in the left lower image). Scale bar = 0.5 cm d. Newborn mice at P1 born to E90-vaccinated mothers. Scale bar = 1 cm. (PDF 92 kb)