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Additional file 3: of Chromatin-wide and transcriptome profiling integration uncovers p38α MAPK as a global regulator of skeletal muscle differentiation

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posted on 2016-03-15, 05:00 authored by Jessica Segalés, Abul Islam, Roshan Kumar, Qi-Cai Liu, Pedro Sousa-Victor, F. Dilworth, Esteban Ballestar, Eusebio Perdiguero, Pura Muñoz-Cánoves
Supplementary Figures 1 and 2 and legends. Figure S1. Relative expression of p38α and p38β in C2C12 cells. The expression of p38α and p38β was measured by qPCR in differentiating C2C12 cells. Values are mean ± SEM of three independent set of samples. Figure S2. Nuclear p38α remains constant in proliferating and differentiating myoblasts. Expression of p38α in C2C12 cells in GM and 24 h in DM: p38α (green) and DAPI (blue). Nuclear to cytoplasm fluorescence ratio was calculated using integrated density values in ImageJ software with DAPI as nuclear boundary. Scale bar = 25 μm. (PDF 7595 kb)

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    Skeletal Muscle

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    DMp 38βp 38αFigure S 1.Figure S 2. Nuclear p 38αDAPIcytoplasm fluorescence ratiointegration uncovers p 38α MAPKPDF 7595 kbC 2C cellsGMmuscle differentiation Supplementary Figures 1

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