Additional file 3: of Characterization of a relaxase belonging to the MOBT family, a widespread family in Firmicutes mediating the transfer of ICEs

Figure S3. Calibration curve of the gel filtration column. A Sephadex S200 HiLoad 16/60 column (GE Healthcare) was used as a final step of purification of RelSt3 protein. This column was calibrated and allowed us to estimate the apparent molecular weight of RelSt3. See complete legend in Additional file 4. (PPTX 40 kb)