Additional file 3: of A novel BMI-1 inhibitor QW24 for the treatment of stem-like colorectal cancer

Figure S3. BMI-1 protein level is higher in cancer cells than normal cells and overexpression of BMI-1 correlates with poor patient survival in colorectal cancer. A, BMI-1 protein levels in various cells were measured by western blotting analysis, including human breast cancer cells MDA-MB-231, lung cancer cells A549, ovarian cancer cells ES2, liver cancer cells HepG2, prostate cancer cells PC3 and DU145, colorectal cancer cells HT29 and HCT116, as well as human normal liver cell L02, human skin fibroblast cell HAF, human normal colon epithelium cell NCM460 and human umbilical vein endothelial cell HUVEC. B, BMI-1 is differently expressed in colorectal cancer and normal tissues, as indicated by UALCAN ( http://ualcan.path.uab.edu ) [76]. C, Higher expression levels of BMI-1 showed poor survival rates in colorectal cancer patients, as indicated by The Human Protein Atlas ( https://www.proteinatlas.org ) [77]. (DOCX 82 kb)