Additional file 3: of A glycine-rich protein MoGrp1 functions as a novel splicing factor to regulate fungal virulence and growth in Magnaporthe oryzae

Figure S2. Targeted deletion of MoGRP1. (A) Schematic diagram showing the strategy of MoGRP1 replacement by the hygromycin resistance gene hyg. The gene knock-out vector of MoGRP1 was constructed by amplifying the flanking sequences with primer pairs koF1/koR1 and koF2/koR2 and were ligated with the hph cassette. (B) DNA gel blot analysis of the wild-type strain P131 and one ΔMogrp1 deletion mutant kg1. The genomic DNAs were digested by XhoI, hybridized with the probe, and amplified with the primer pair koF1/koR1. The estimated size of each band is labeled on the left. (TIF 402 kb)