Additional file 3: Table S3. of Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis

Annealing/extension temperature determination to the standardization of qPCR assay for the parasite load quantification. To obtain the Ct values, the threshold was set at 0.02 in all assays. (DOC 31 kb)