Additional file 3: Figure S3. of Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

MacConaill, Laura; Burns, Robert; Nag, Anwesha; Coleman, Haley; Slevin, Michael; Giorda, Kristina; Light, Madelyn; Lai, Kevin; Jarosz, Mirna; McNeill, Matthew; Ducar, Matthew; Meyerson, Matthew; Thorner, Aaron

doi:10.6084/m9.figshare.5768856.v1

12864_2017_4428_MOESM3_ESM.pdf (936.59 kB)

Additional file 3: Figure S3. of Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

journal contribution

posted on 2018-01-08, 05:00 authored by Laura MacConaill, Robert Burns, Anwesha Nag, Haley Coleman, Michael Slevin, Kristina Giorda, Madelyn Light, Kevin Lai, Mirna Jarosz, Matthew McNeill, Matthew Ducar, Matthew Meyerson, Aaron Thorner

Schematic of the sources of sample cross-talk including adapter contamination, index hopping during multiplex target enrichment post-capture PCR, index hopping during cluster amplification, and demultiplexing errors. Unique dual-matched indices reduce read misassignment because unexpected index combinations are removed from downstream analysis. (PDF 936 kb)