Additional file 3: Figure S3. of Unique, dual-indexed sequencing adapters with UMIs effectively eliminate index cross-talk and significantly improve sensitivity of massively parallel sequencing

Schematic of the sources of sample cross-talk including adapter contamination, index hopping during multiplex target enrichment post-capture PCR, index hopping during cluster amplification, and demultiplexing errors. Unique dual-matched indices reduce read misassignment because unexpected index combinations are removed from downstream analysis. (PDF 936 kb)