12986_2017_235_MOESM3_ESM.docx (56.59 kB)
Additional file 3: Figure S3. of The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism
journal contribution
posted on 2018-01-22, 05:00 authored by Jing Tian, Jiapan Liu, Jieqiong Li, Jingxin Zheng, Lifang Chen, Yujuan Wang, Qiong Liu, Jiazuan NiThe reductase activity of SELENOFâ toward all-trans-retinaldehyde. The plasmid SELENOFâ-PET28b was transfected into E.coli (BL21) and recombinant SelenoFâ was induced and then the cell lysates were used to detect the enzyme activity to reduce all-trans-retinaldehyde (column A); Empty vector PET28b was transfeced into E.coli (BL21) and used as the negative control (column B). (DOCX 53 kb)