Additional file 3: Figure S3. of The human Na+/H+ exchanger 1 is a membrane scaffold protein for extracellular signal-regulated kinase 2

Analysis of the hNHE1cdt-iaERK2 complex by size exclusion chromatography and circular dichroism (CD). (a) Size exclusion chromatography profiles of the hNHE1cdt WT (blue), iaERK2 (black), and a mixture of both (red). Subtraction of the individual runs from the mixture identifies a broad underlying peak with elution properties of the complex (grey). (b) Zoom from panel (a). Fractions taken for SDS-PAGE analysis (see panel (c)) are indicated by grey bars. (c) Coomassie-stained SDS-PAGE of fractions from individual runs, as indicated in panel (b). (d) Mean residual ellipticity CD spectra of hNHE1cdt WT, iaERK2, and mixture reveal no significant gain of secondary structure upon complex formation. (PDF 5632 kb)