Additional file 3: Figure S3. of Temperature-dependent changes in the host-seeking behaviors of parasitic nematodes

2016-05-06T05:00:00Z (GMT) by Joon Lee Adler Dillman Elissa Hallem
Temperature-induced changes in sensory valence are consistent across CO2 and odorant concentrations in Steinernema carpocapsae. A. Responses of infective juveniles (IJs) to various CO2 concentrations. CO2 was attractive for IJs across concentrations regardless of age or temperature treatment, although 25 °C IJs showed an increased response to 0.5 % CO2 at day 14 compared to day 0. * P < 0.05 relative to day 0 IJs; two-way ANOVA with Dunnett’s post-test; n = 6–10 trials for each condition. B. Responses to different concentrations of 1-hexanol and 2-propanone were compared for day 0 IJs maintained at 25 °C, day 14 IJs that were temperature-swapped from 25 °C to 15 °C on day 0, and day 14 IJs maintained at 25 °C. Responses of consistent valence were observed across concentrations. Data for undiluted concentrations are from Fig. 3. Responses of 25 °C IJs to 2-propanone were significantly different between day 0 and day 14 at 10–4 (P < 0.05) and 10–5 (P < 0.01) concentrations in a two-way ANOVA with Sidak’s post-test, comparing 100 through 10–6 dilutions between 25 °C day 0 and 25 °C day 14 IJs; n = 6–22 trials for each condition. C. IJs exhibit olfactory plasticity in response to odor blends. IJs were tested for their response to three different mixes of odorants. Mix 1 contained 10–1 dilutions of methyl acetate, 1-propanol, and 2-propanone; Mix 2 contained 10–1 dilutions of 1-hexanol, 6-methyl-5-hepten-2-one, and methyl salicylate; and Mix 3 contained 10–1 dilutions of 1-hexanol, 7-octenoic acid, and 2,2,4-trimethylpentane. *** P < 0.001 relative to day 0 IJs; two-way ANOVA with Dunnett’s post-test; n = 8 trials for each condition. For all graphs, error bars represent standard error of the mean (SEM). Mean, n, and SEM values for each assay are listed in Additional file 7: Dataset S1. (PDF 428 kb)