Additional file 3: Figure S3. of RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures

Correlation of read counts. The scatter plots show the correlation between read counts in VIM + −sorted material and PC3 cells (a) and the correlation between read counts in the heterogeneous Raji:PC3 population and Raji cells (b) for the differentially expressed genes in Fig. 4a. The red line is a linear fit to the data. ρ indicates the Pearson’s correlation coefficient. The data is plotted as log2 of the average read count (in counts per million, CPM) normalized for library size. The green data points in (a) represent a subset of the 23 % (1057) of differentially expressed genes that were unique to the VIM+ PACS sort vs. pure Raji comparison from the Fig. 4b Venn diagram. The blue data points were also identified exclusively in the pure PC3 vs. pure Raji comparison (998 genes shown in Fig. 4b). (TIF 14209 kb)