Additional file 3: Figure S3. of Acute maternal oxidant exposure causes susceptibility of the fetal brain to inflammation and oxidative stress

Intracellular GSH is pivotal in EtOH-induced phenotypic acquisition of microglia. EOC 13.31 cells grown on coverslips were treated with NAC (500 μM) or BSO (200 μM) for 18 h prior to the presence or absence of EtOH (22 mM) exposure for 6 h. Cells were labeled by antibodies against iNOS and Alexa Fluor 488 labeled secondary antibodies. The nuclei of the cells were counterstained with DAPI. GSH depletion by BSO and EtOH exposure synergistically exaggerate the expression of M1 marker (green) iNOS. Images were acquired on FV1000 confocal microscope equipped with a HeNe laser, 60× objective, NA 1.42 with an electronic zoom of 2. Scale bar = 40 μm (TIFF 301 kb)