Additional file 3: Figure S2. of uPAR enhances malignant potential of triple-negative breast cancer by directly interacting with uPA and IGF1R

Combined RNAi of uPAR and IGF1R significantly reduced the tumourigenic potential of TNBC cells. a: Representative Western blot analysis of uPAR and IGF1R following RNAi using three different siRNAs per target protein, respectively and of (b) MMP2 and MMP9. Tubulin was used as loading control. c: In vitro viability assays (n = 5), d: scratch wound assays (n = 3) and (e) matrigel invasion assays (n = 3) are shown 48 h post-transfection. siRNAs for transient downregulations of target proteins or of GAPDH (positive control) and a non-targeting siRNA as negative control were used in triplicates according to previous protocol [37]. f: Relative mRNA levels of uPAR, IGF1R and uPA following stable RNAi of target proteins determined by qRT-PCR (n = 3) are shown. The quantifications were determined in relation to mock control. Standard deviations and p-values are shown. (PDF 336 kb)