Additional file 3: Figure S1. of TLR7-mediated skin inflammation remotely triggers chemokine expression and leukocyte accumulation in the brain

Transcriptional PBL response of Aldara-treated mice. Mice were treated with 80 mg Aldara cream or control cream every 24 h for 1, 3 or 5 consecutive days. Mice were euthanised 24 h after the final application. Cardiac puncture was performed to retrieve PBLs and RNA was isolated from the tissue. (A) QRT-PCR analysis of the target chemokine genes was performed. n = 4 mice per group. Significance was measured using two-way ANOVA with Bonferroni multiple comparison post-tests ***p ≤ 0.001 **p ≤ 0.01 *p ≤ 0.05. (PNG 32 kb)