Additional file 3: Figure S1. of Marked differences in tight junction composition and macromolecular permeability among different intestinal cell types

Immunofluorescence of the stem cell marker CD44 in organoids enriched in stem cells (ISCs), enterocytes (ENTs), goblet cells (GOBs), and Paneth cells (PANs). CD44 staining is shown in red, while nuclei are blue. Notice the intense staining of CD44-positive cells in ISC organoids. There was virtually no CD44 in ENT and GOB organoids. Modest levels of CD44 were located in PAN organoids where some stem cells are found. (PDF 351 kb)