Additional file 3: Figure S1. of An oncogenic mutant of RHEB, RHEB Y35N, exhibits an altered interaction with BRAF resulting in cancer transformation

RHEB Y35N Does not Exhibit Increased Binding to AMPK. A) RHEB WT, T38A, and Y35N mutants were transiently transfected and expressed in HEK 293T cells, cell lysates were collected, and immunoprecipitation for each was carried out. These results show a Western blot for AMPKÎą and FLAG from those samples. An effector domain mutant, RHEB T38A, did not bind AMPK demonstrating that AMPK is a relevant effector of RHEB (PDF 154 kb)